In Vitro Studies on Callus Induction of Mimusops Elengi L. (Bakul)
Sherkar Sandip Harishchandra and Tabe R. H.
VSBT’s College of Agriculture Biotechnology, Vidyanagri, Baramati-413133
(Affiliated To Mahatma Phule Krushi Vidyapith, Rahuri, Dist-Ahmednagar)
Article Info |
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Abstract |
Received: 22-11-2019, |
A rapid highly efficient protocol was developed for inducing callus using different explants of Mimusops elengi L. (Bakul) viz., node, internode and leaves. The results of in vitro callus development using different explants and growth regulators from Mimusops elengi were studied. Explants were collected from field grown M. elengi plant which was in flowering stage. The highest efficiency of surface sterilization treatments for nodal segments (95.0 %), intermodal (92.33%) and leaves (85.0%) were observed when the explants surface sterilized with Sodium hypochlorite 4% for 20 min and subsequently treated with 0.01% HgCl2 for 5 min. The explants were cultured on Murashige and Skoog (MS) basal semisolid medium with various concentrations of 2, 4-Dichloro phenoxy acetic acid (2, 4-D) alone and in combinations with Naphthalene acetic acid (NAA), were used for callus induction. All the explants showed positive response for callus induction. In addition to the concentration of plant growth regulator, the type of explant also had an effect on callus induction. Maximum response for callus induction was observed, when nodal segments and leaf discs inoculated on MS medium supplemented with 2.0 mg/l 2, 4-D and 1.5 mg/l NAA (97% and 96.33%, respectively). Internodal segments shows maximum callus formation (70.66%) on MS medium supplemented with 2.0 mg/l 2, 4-D and 1.5 mg/l NAA with good callus growth. In our experiment we found nodal and leaves was most suitable explants for in vitro callus induction and growth. MS medium supplemented with 2, 4-D and NAA can be used for the callus induction of Mimusops elengi L. |
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Keywords: Bakul, Callus Induction, Explants, MS medium, 2, 4-D, NAA. |
REFERENCES
Ali MA, Mozid MA, Yeasmin S, Khan AM and Sayeed MA, 2008. An evaluation of antimicrobial activities of Mimusops elengi Linn. Research Journal of Agriculture and Biological Sciences. 4(6):871-874.
Baliga MS, Pai RJ, Bhat HP, Palatty PL and Boloor R, 2011. Chemistry and medicinal properties of the Bakul (Mimusops elengi Linn): A review. Food Research International, 44(7):1823-1829.
Bhore SJ and Preveena J, 2011. Micropropagation of Mimusops elengi Linn. identification of suitable explant and comparative analysis of immature zygotic embryos response on three basal media. American-Eurasian Journal Agriculture and Environmental Science, 10(2):216-222.
Boonyuen C, Wangkarn S, Suntornwat O and Chaisuksant R, 2009. Antioxidant capacity and phenolic content of Mimusops elengi fruit extract. Kasetsart Journal (Natural Science), 43(1):21-27.
Daud NH, Jayaraman S and Mohamed R. 2012. Methods Paper: An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilaria malaccensis from field sources into tissue culture. Asia-pacific Journal of Molecular Biology Biotechnology, 20(20):55-58.
Gami B, Parabia M and Kothari IL. 2010. In vitro development of callus from node of Mimusops elengi- as substitute of natural bark. International Journal of Pharmaceutical Science and Drug Research, 2(4): 281-285.
Gomez KA and Gomez AA, 1984. Statistical procedures for agricultural research. John wiley and Sons, New York. pp. 357- 427.
Gopitha K, Bhavani AL and Senthilmanickam J, 2010. Effect of the different auxins and cytokinins in callus induction, shoot, root regeneration in sugarcane. International Journal Pharma and Bio Science,1(3):1-7.
Hong TD and Ellis RH. 1996. A Protocol to determine seed storage behavior. IPGRI Technical Bulletin No-1. International Plant Genetic Resources Institute, Rome. p. 64.
Hussey G. 1986. Vegetative propagation of plants by tissue culture. Plant cell culture technology. Yeoman MM (ed), Blackwell Scientific Publications, Oxford. pp.29-66.
Kusumoto IT, Nakabayashi T, Kida H, Miyashiro H, Hattori M, Namba T and Shimotohno K, 1995. Screening of various plant extracts used in ayurvedic medicine for inhibitory effects on human immunodeficiency virus type 1 (HIV-1) protease. Phytotherapy Research, 9(3):180-184.
Lemmens RHMJ. 2005. Mimusops elengi L. PROTA (Plant Resources of Tropical Africa / Ressources vegetales de l Afrique tropicale). Louppe, D, Oteng-Amoako AA and Brink M (eds). Wageningen, Netherlands).
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bio assay with tobacco tissue cultures. Physiologia Plantarum. 15(3):473-497.
Skoog F and Miller C, 1957. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposia of the Society for Experimental Biology,11: 118-131.
How to cite this article
Sherkar Sandip Harishchandra and Tabe R. H., 2020. In Vitro Studies on Callus Induction of Mimusops Elengi L. (Bakul). Bioscience Discovery, 11(2):76-84.
Google Scholar citation: https://scholar.google.co.in/citations?user=vPzEyC8AAAAJ&hl=en
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