Bioscience Discovery, Volume 10, Number 4, Page Number 214-221, Month & Year: Oct. - 2019

Spectrum and frequency of Chlorophyll mutation in grass pea (Lathyrus sativus L)

Kulkarni Ganesh. B.

Associate professor in Botany, J.E.S. R.G. Bagdia Arts, S.B. Lakhotia Commerce and
R. Bezonji Science College, Jalna  431203 (M.S.), India
E-mail: [email protected]

 

Article Info

 

Abstract

Received: 25-06-2019,
Revised: 10-08-2019,
Accepted: 26-08-2019

The seeds of grass pea (Lathyrus sativus L.) various chemical mutagen concentrations (EMS 0.05%, 0.10%, 0.15%; SA 0.005%, 0.010%, 0.015% and  NMU 0.15%, 0.30%, 0.45%) and physical mutagen doses (Gamma rays- 15KR, 30KR, 45KR) were treated in laboratory. The Treated and untreated seeds were sown in the field by CRBD method. The growth of M1 generation observed the characters and number of mutants in each population thoroughly. The chlorophyll mutation frequency was calculated on the basis of plant population with explicit chlorophyll mutation and leaf phenotypic diversity.  A noticeable varied range of chlorophyll and morphological mutants were observed in the M1 generations. Distinct kinds of spectrum of chlorophyll mutants’ viz. xantha, viridis, albino and chlorina were identified in the treated population. Prominent phenotypic aberrations were also noted viz. reduction in size of leaves, obtuse and retuse apex, notch at margin, bilobed leaf, lanceolate leaf, wrinkled leaflet and triforked leaf, etc. The frequency of leaf morphological changes observed in almost all chemical and physical mutagen in grass pea.

Keywords:
Grass pea (Lathyrus sativus L.), EMS, SA, NMU, Gamma rays, chlorophyll mutation, leaf aberrations.

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How to cite this article

Kulkarni Ganesh. B., 2019. Spectrum and frequency of Chlorophyll mutation in grass pea (Lathyrus sativus L).. Bioscience Discovery, 10(4):214-221.

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