In vitro synergism between algae and bacteria isolated from bio-diversity hotspot for better environmental sustainability
Debapriya Roy*, Srijan Bhattacharya, Antara Biswas, Arpan Banerjee, Shinjini Ghosh and Arup Kumar Mitra
Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata.
*[email protected]
Article Info |
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Abstract |
Received: 22-03-2019, |
A water sample containing algae was collected from Manipur. Microscopic examination revealed that it contained filamentous green algae and diatoms. Upon pour plating it gave rise to three types of bacterial colonies which were Bacillus spp, Staphylococcus spp and Streptomyces spp. To artificially prepared BG11 broth respective proportions of algae and bacterial cultures were inoculated followed by constant monitoring of algal biomass after 15 and 30 days accordingly. Each of the bacteria promotes algal growth as indicated from the in-vitro increase in the algal biomass with Bacillus spp promoting the maximum growth. The symbiotic interaction between Bacillus spp and the algae resulted in the increase of the algal biomass by 10.71%. So, on a detailed study under SEM, it was found out that the algal sample procured nourishment from the nutrients supplied by the Bacillus spp as well as the glycocalyx adhesion of Bacillusspp with that of cellulosic algal wall. On the other hand, Chlorophyll assay and Carotenoid assay was performed which revealed the ability of the algae to perform photosynthesis with the help of increased Chlorophyll b and production of Carotenoid. The measurement of Chlorophyll a and b at 645nm and 663nm revealed the increase of Chlorophyll b concentration by 3.05%, along with increase in Carotenoid concentration at 470nm was determined to be 0.97µg/ml. The artificial cultivation of diatoms resulted in partial degradation of silica wall which was replenished by bacterial conversion of dead debris of diatoms. Thus it can be concluded that the bacterial association not only facilitate nutrient availability but also helps in partial increase in anabolic process of the algal consortia. |
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How to cite this article
Debapriya Roy, Srijan Bhattacharya, Antara Biswas, Arpan Banerjee, Shinjini Ghosh and Arup Kumar Mitra, 2019. In vitro synergism between algae and bacteria isolated from bio-diversity hotspot for better environmental sustainability. Bioscience Discovery, 10(3):134-141.
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