PHB production from bacteria isolated from oil contaminated soil
Nausheen Sadaf1 and D. C. Kamthane2
1Research student, NSB College, Nanded. E mail: [email protected]
2Head, Dept. of Microbiology, SGB College, Purna.
E mail: [email protected]
Article info
Received: 01-02-2019,
Revised: 03-04-2019,
Accepted: 08-04-2019
Abstract
PHB was prepared from the bacterial species isolated from oil contaminated soil. Five efficient producers were proceeded for PHB production. Two crude carbon sources, groundnut oil cake powder and molasses were tested versus pure sugar glucose as carbon source for production. The isolates were biochemically characterized by Bergey’s Mannual of Systematic bacteriology 2nd edition 2001. The produced PHB was confirmed by Spectrophotometric method and FTIR. Better production was observed with groundnut oil cake powder as compared with pure glucose. Optimum pH, temperature and incubation time for PHB production was also determined.
Keywords
Bergey’s Mannual of Systematic Bacteriology, carbon source, groundnut oil cake powder, molasses, PHB.
K. Susithra, U. Ramesh. Kannan, R. Ganesan and K. Rajarathinam, 2017, Screening and characterization of bioplastics producing bacteria isolated from oil contaminated soils of Virudhunagar, Tamil Nadu, India. Pharmaceuticlas and Biological evaluations, 4 (1): 21-27.
Reddy Vishnuvardhan and M.Thirumala, 2012, Isolation of polyhydroxyalkanoates (PHA) producing bacteria from contaminated soils. International Journal of Environmental Biology, 2(3): 104-107.
Sajida Munir and Nazia Jamil, 2015, Characterization of Polyhydroxyalkanoates Produced by Contaminated Soil Bacteria using Wastewater and Glucose as Carbon Sources. Tropical Journal of Pharmaceutical Research, 14 (9): 1605-1611.
Shenoy Sushma, Mascarenhas Joyline and K. Aruna, 2012, Optimization of PHB accumulation by Klebsiella SP NCCP-138 isolated from oil contaminated soil. International Journal of Pharma and Bio Sciences, 3(4): (B) 559 – 570.
T. Rawte and S.Mavinkurve, 2002, A rapid hypochlorite method for extraction of polyydroxy alkanoates from bacterial cells. Indian Journal of Experimental Biology. 40(1):924-929.
How to cite this article
Nausheen Sadaf and D. C. Kamthane, 2019. PHB production from bacteria isolated from oil contaminated soil. Bioscience Discovery, 10(2):103-107.
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