In Vitro Studies on Callus Induction of Mimusops Elengi L. (Bakul)
Sherkar Sandip Harishchandra and Tabe R. H.
VSBT’s College of Agriculture Biotechnology, Vidyanagri, Baramati-413133
(Affiliated To Mahatma Phule Krushi Vidyapith, Rahuri, Dist-Ahmednagar)
A rapid highly efficient protocol was developed for inducing callus using different explants of Mimusops elengi L. (Bakul) viz., node, internode and leaves. The results of in vitro callus development using different explants and growth regulators from Mimusops elengi were studied. Explants were collected from field grown M. elengi plant which was in flowering stage. The highest efficiency of surface sterilization treatments for nodal segments (95.0 %), intermodal (92.33%) and leaves (85.0%) were observed when the explants surface sterilized with Sodium hypochlorite 4% for 20 min and subsequently treated with 0.01% HgCl2 for 5 min. The explants were cultured on Murashige and Skoog (MS) basal semisolid medium with various concentrations of 2, 4-Dichloro phenoxy acetic acid (2, 4-D) alone and in combinations with Naphthalene acetic acid (NAA), were used for callus induction. All the explants showed positive response for callus induction. In addition to the concentration of plant growth regulator, the type of explant also had an effect on callus induction. Maximum response for callus induction was observed, when nodal segments and leaf discs inoculated on MS medium supplemented with 2.0 mg/l 2, 4-D and 1.5 mg/l NAA (97% and 96.33%, respectively). Internodal segments shows maximum callus formation (70.66%) on MS medium supplemented with 2.0 mg/l 2, 4-D and 1.5 mg/l NAA with good callus growth. In our experiment we found nodal and leaves was most suitable explants for in vitro callus induction and growth. MS medium supplemented with 2, 4-D and NAA can be used for the callus induction of Mimusops elengi L.
Keywords: Bakul, Callus Induction, Explants, MS medium, 2, 4-D, NAA.
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How to cite this article
Sherkar Sandip Harishchandra and Tabe R. H., 2020. In Vitro Studies on Callus Induction of Mimusops Elengi L. (Bakul). Bioscience Discovery, 11(2):76-84.
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