Influence of AM Fungi, Azotobacter and Phosphate Solubilizing Bacteria on Withania somnifera (L.) Dunal.- A venerable medicinal plant
H. C. Lakshman1, Channabasava A2*. and K. P. Kolkar3
1Post graduate Department of studies in Botany, Karnatak University Dharwad- 580003, India.
2Department of Botany, Sri Gavisiddeshwar Arts, Science and Commerce College, Koppal-583231, Karnataka
3Department of studies in Botany, Karnatak Science College Dharwad- 580001, India
Arbuscular mycorrhizal fungal (AMF) interaction with other microorganisms on the Withania somnifera (L.) Dunal, was carried out under the greenhouse condition and their beneficial impacts were discussed. There is a contradictory statement of mycorrhizal colonization in solanaceae; therefore an attempt was made to explore mycorrhizal association with AM fungus (Glomus macrocarpum), Azotobacter and Phosphate solubilizing bacteria (PSB). Green house experiments on Withania somnifera was carried out with following treatments (1) control (non-inoculated) (2) Glomus macrocarpum (3) Glomus macrocarpum+ Azotobacter (4) Glomus macrocarpum + PSB (Bacillus subtilis) (5) Glomus macrocarpum + Azotobacter + PSBin which triple inoculation (Glomus macrocarpum + Azotobacter + PSB) shows higher shoot, root length and dry weight of shoot and root followed by dual and single inoculation compared to non-inoculated plants. The experimental plants revealed higher in per cent of root colonization, spore number and P uptake of shoot, after triple inoculation compared to control plants. The result suggests that there is a synergistic interaction with different bio-inoculants and the increase of biomass with the application of these bio-inoculants.
Keywords: Solanaceae, Withania somnifera, bio-inoculants, Glomus macrocarpum, Azotobacter, Phosphate solubilizing bacteria (PSB), Bacillus subtilis.
Antones V and Cardon EJBN, 1991. Growth and nutrient status of citrus plants as influenced by mycrorrhiza and phosphorus application. Plant and Soil, 31: 11-15.
Borea JM and Jeffries P, 1995. Arbuscular mycorrhizae in sustainable and plant systems. In Mycorrhiza structure, function, molecular biology and biotechnology , Hock B. and Varma A., eds., Springer-Verlag, Heidelberg, Germany, 521-559.
Borea JM, Azcon R and Azcon-Aguilar C, 1983. Interactions between phosphate solubilizing bacteria and VA mycorrihiza to improve the utilization of rock phosphate in nonacidic soils. In proceeding of the 3rd International Congress of Phosphorus compounds, Institute Mondial du Phosphate, Casablanca, 127-144.
Bushan Y and Hoglguin G, 1998. Proposed for the division of plant growth promoting rhizobacteria into two classification, biocontrol-PGPB (Plant Growth Promoting Bacteria) and PGPB, Soil Biochem, 30: 1225-1228.
Negalur CB and Lakshman HC, 2016. Interaction studies between different AM fungi and PSB on growth, chlorophyll and lipid content. In: Proceedings of Interactional conference on green technology for sustainable Ecosystems, Feb. St. Joseph’s college Bangalore. India, Pp. 163-171.
Dobbelaers S, Vanderleyden J and Okon YY, 2003. Plant growth promoting effects of diazotrophs in the rhizosphere. Crit. Rev. Plant Sci., 22:107-149.
Durga VVK and Gupta S, 1995. Effect of vesicular arbuscular mycorrhizae on the growth and mineral nutrition of Teak (Tectona grandis Linn. F). Indian forester, 121(6): 518-527.
Gaind S, Rathi MS, Kaushik BD, Nain L, Verma OP, 2007. Survival of bio-inoculants on fungicides-treated seeds of wheat, pea and chickpea and subsequent effect on chickpea yield. J Environ Sci Health B, 42: 663-68.
Galleguillos C, Aguirre C, Barea JM and Azcon R, 2006. Growth promoting effect of two Sinorhizobium meliloti strains (a wild type and its genetically modified derivative) on a non-legume plant species in specific interaction with two arbuscular mycorrhizal fungi. Plant Sci., 159: 57-63.
Gerdemann JW and Nicolson TH, 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting technique, Trans of the British Mycol. Soc. 46: 235-243.
Harley JI and Harley FI, 1987. A Checklist of mycorrhiza in the British Flora, New Phytol., 105(2): 1-102.
Jackson ML, 1973. Soil Chemical Analysis, Prentice Hall, New Delhi, 368 pp.
Kiers TE, Duhamel M, Beesetty Y, Mensah JA, 2011. Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis. Science, 333: 880–882.
Lakshman HC, 2014. Synergistic effect between PSB and arbuscular mycorrhizal fungi on growth and phosphorus uptake in Niger plants Guizatia abyssinica (L. f) Cass. Research J. of Agri. Sci., 5(2): 185-187.
Lakshman HC, 2015. Plant growth promoting rhizobacteria (PGPR): mediating and facilitating microorganisms for plant sustainable in soil. In: Major constrains and verdict of crop productivity. Ed. U. N. Bhale. Astral International (P) limited New Delhi. India. Pp. 170-181.
Lakshman HC, 2009. AM Fungi a promising bioinoculation for sustainable plant growth. In proceeding of ICAR conference, NAL Bangalore, April, 14th pp, 117-121.
Lakshman HC, Mulla FI, Inchal RF and Srinivasalu S, 2001. Prevalence of Arbuscular mycorrhizal fungal colonization in some disputed plants, Mycorrhizal News, 13(3): 17-21.
Marschner H and Dell B, 1994. Nutrient Uptake in mycorrhizal symbiosis, Plant and Soil, 159: 89-102.
Naik RR and Lakshman HC, 2010. Effect of AM Fungi PGPR and different soil nitrogen sources to improve growth and yield of Paddy (cv. JAYA), Inter.J. Plant. Sci., 5(1): 165-169.
Nautiyal CS, Mehta S and Singh HB, 2006. Biological control and plant growth promotion by Bacillus strains from milk. J. Microbial, Biotechnol., 16:184-192.
Neeraj AS, Mathew J and Varma A, 1991. Occurrence of vesicular arbuscular my corrhizae with Amarathaceae in soils or the Indian semi and region. Biol. Fertile Soil, 11: 140.144.
Parmar N, 1995. Interactions of rhizosphere bacteria with Cicer-Rhizobium symbiosis. Hisar, India: CCS Haryana Agricultural University.
Peterson RL, Ashford AE and Allaway WG, 1985. Vesicular arbuscular mycorrhizal association of vesicular plants on Heron islands, a Great Barrier Reef coral ray, Aust. J. Bot., 33: 669-676.
Phillips JM and Hayman DS, 1970. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans of the Brit. Mycol. Soc., 55: 158-161.
Poonguzhali S, Madhiyan M and Sa TM, 2008. Isolation and identification of phosphate solublizing bacteria from Chinese cabbage and their effect on growth and phosphasarus utilization of plants. J. Micro. Biol. Biotechnol, 18: 773-777.
Sandeepkumar K, Lakshman HC and Channabasava A, 2011. Influence of plant growth promoting microorganisms and AM fungi on some important fiber yielding plants. Bulletin of Basic and Applied plant Biology, 1:41-48.
Smith SE and Read DJ, 1997. Mycorrhizal Symbiosis, Academic Press, London, 60pp.
Sujata D and Nibha G, 2011. Microbial bioinoculants and their role in plant growth and development. International Journal for Biotechnology and Molecular Biology Research, 2(13): 232-251.
Suman DA, Bagyaraj DJ and Arpana J, 2003. Interaction between Glomus mosseae, Azotobacter Chooococcum and Bacillus coagulans and their influence on growth and nutrition of Neem. J. Soil Biol. Ecol., 23:80-86.
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HC Lakshman, Channabasava A and KP Kolkar, 2020. Influence of AM Fungi, Azotobacter and Phosphate Solubilizing Bacteria on Withania somnifera (L.) Dunal.- A venerable medicinal plant. Bioscience Discovery, 11(1):01-06.
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