Antagonistic Capability combined Trichoderma harzianum and T. Pseudokoningii against Alternaria alternata (Fries ) Keissler causing leaf spot of Gerbera
Waghmare M. B.
Department of Advanced Research in Botany,
The New College, Kolhapur -416012.Maharashtra, India
Gerbera is the ornamental plant suffering from the leaf spot caused by Alternaria alternata. Present ecofriendly studies have confirmed the importance and effectiveness of the Trichoderma species against many fungal phytopathogens In the present study investigation has been made on the bio control effects of Trichoderma harzianum alone ,in combination with Trichoderma pseudokoningii and alternate use were evaluated against the Alternaria alternata causing leaf spot of gerbera. In the continuous passage Trichoderma harzianum used alone against the pathogen up to seventh passage. In alternate passage Trichoderma harzianum used alternately with T. pseudokoningi and in the Mixed passage both the Trichoderma Sp. used combindly and Screened against the pathogen by dual culture technique. Among these, alternate and combined used of Trichoderma sp. shown the significant reduction in the growth of the pathogen. 84.33% and 78.66 % of growth inhibition was found in the combined and alternate use Trichoderma harzianum. While in continuous passage or alone use increased the Trichoderma harzianum resistance in the Pathogen.
Barnett HL and Hunter BB, 1972. Illustrated Genera of Imperfect Fungi, Burgess Publishing Co. Minneapolis, Mn, USA, pp. 241.
Bhai RS and Thomas J, 2010. Compatibility of Trichoderma harzianum (Rifai.) with fungicides, insecticides and fertilizers. Indian Phytopathology. 2010; 63(2):145-148
Bikila Wedajo, 2015. Compatibility Studies of Fungicides with Combination of Trichoderma Species under In vitro Conditions. Virology and Mycology, 2: 2-5.
Elad Y, Zimmand G, Zags Y, Zuriel S, Chet I, 1993. Use ofTrichoderma harzianum in combination or alternation with fungicides to control Cucumber grey mold (botrytis cinerea) under commercial greenhouse condition. Plant Pathol. 42: 324-356.
Gams W, Bissett J, 1998. Morphology and identification of Trichoderma. In: Harmann, G. E., Kubicek, C. P. (eds.) Trichoderma and Gliocladium. Taylor and Francis, London. 3-34
Gangawane LV, 1990. Fungicide resistance in plant pathogen in Malaysia Pub. pp. 117-121. Indian Phytopathology, 40:551-553.
Johnson LA, 1957. Effect of antibiotics on the number of bacteria and fungi isolated from soil by dilution plate method. Phytopathology, 47: 21-22.
Mohammad Akrami1, Hadi Golzary and Masoud Ahmadzadeh, 2011. Evaluation of different combinations of Trichoderma species for controlling Fusarium rot of lentil. African Journal of Biotechnology, 10(14): 2653-2658,
Mohanan C, 1996. Epidemiology and control of Rhizoctonia web blight of bamboo’. Proceedings of Diseases of Insect Pests in tropical Forests. (Eds.). Nair, K.S.S.; Sharma, J.K. and Varma, V.V. Peechi, India: Kerala forest Research Institute. 169-185
Morton DJ and Stroube WH, 1955 . Antagonistic and stimulating effects of soil micro-organism of Sclerotium. Phytopathol., 45:417-420
Priti Sonavane and Venkataravanappa, 2017. Compatibility Studies of Trichoderma harzianum Isolate with Fungicides used against Soil Borne Disease in Coorg Mandarin-Pepper-Coffee Plantations. Int. J. Curr. Microbiol. App.Sci., 6(8): 346-354
Nagamani A and Manoharachary C, 2002. Monographic Contribution on Trichoderma. Associated Publishing Company. New Delhi.
Ricker AJ and Ricker RS, 1936. Introduction to Research on Plant diseases John S. Swift Co., St. Louis, pp 117.
Subramanian CV, 1971 . Hypomycetes an account of Indian species except Cercospora. Indian Council of Agricultural Research, New Delhi, pp 810
Tapwal A, Kumar S, Gautam N, Pandey S, 2012. Compatibility of Trichoderma viride for Selected Fungicides and Botanicals. International Journal of Plant Pathology, 3: 89-94
Vincent, J. M., 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 150:850.
How to cite this article
Waghmare MB, 2019. Antagonistic Capability combined Trichoderma harzianum and T. Pseudokoningii against Alternaria alternata (Fries ) Keissler causing leaf spot of Gerbera. Bioscience Discovery, 10(4):165-167.
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