Bioscience Discovery, Volume 10, Number 4, Page Number 156-164, Month & Year: Oct. - 2019

Positive effect of Am fungi and pgpr on growth Biomass yield of (sunflower) Helianthus annus Lsh1 var.

Kamble S. S. and H. C. Lakshman

Department of Botany, Shivaji University, Kolhapur– 416 004, (MS).
P.G. Department of Studies in Botany (Microbiology Laboratory)
Karnatak University, Dharwad – 580003, INDIA.

Article Info

 

Abstract

Received: 15-07-2019,
Revised: 22-09-2019,
Accepted: 24-09-2019

Microbial inoculants on plant growth and biomass improvement have been well documented in recent days on majority of  crop  and trees.  In the present work the positive effect of AM Fungi such as; Rhizophagus fasciculatus, Glomus macrocarpum, Glomus bayagyarajii and Sclerocystis dussii with combinatiohn of PGPR Bacillus polymyxa have studied on Helianthus annus L. (sunflower) LSH1.Among the AM fungi Solerocystis dussii combined with Bacillus polymyxa had significancantly influenced on shoot length, root length, stemwidth, dry weights of shoots and roots, per cent root colonization, spore number, Chlorophyll content on leaves, number of seeds per plant, lipid content in seeds and proline accumulation in then roots compared non-inoculated (Control) plants.The second most effective AM Fungus is Rhizophagus fasciculatus with B. Polymyxa. The third most effective influenced AMF is Glomus macrocarpus with B. Polymyxa and the least influenmced AMF is Glomus bagyarajii  with B. Polymyxa.  However, there was favourable increase in all growth parameters over the non-inoculated (Control) plants.  Therefore,  it can concluded that there is synergistic interaction between two microbial inocualation lead to positively improved on Heliantus annus L.

Keywords:
AMF (arbuscular mycorrhizal fungi). Helianthus annus, percent colonization, spore number, Rhizophus fasciculatus, Glomus macrocorpus, Glomus baggarajii and Slerocystis dussii.

REFERENCES
Andress PP, de-Souza R, Granada CE & Passaglia LMP, 2015. Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated. I South Brazil. Biota Neotropica. 15(2): 1590-1676.
Artursson V, Finlay RD and Jansson JK, 2006. Interactions between arbuscular mycorrhizal fyungi and bacteria and their potential for stimulating plant growth. Environ. Microbial., 8: 1-10.
Atkinson D, Watson CA, 2000. The beneficial rhizosphere: a dynamic entity. Applied Soil Ecology, 15:99-104.
Barea Azcon J.M.R. and Hayman DS, 1975. Possible synergistic interactions between  Endogone and phosphate solubilizing bacteria in low phosphates soils.In; Endomycorrhizas (eds.) Sanders, Mosse, F.E. B. and Tinker, P.B. Academic  Press, London, 408 pp.
Barea JM, AF and Oliveres J, 1983. Interaction  between Azospirrillum and V.A. mycorriza and their nutrition of maize and Ryegrass. Soil Biology and Biochemistry, 15:705-709.
Baset MA, and Shamsuddin ZH, 2010. Rhizobium as a crop enhancer and biofertilizer for increased cereal production. Afr. J. Biotechnol.9:6001-6009.
Cardoso,M.j.b. Vasconcellos, R.L.F., Ribeiro, C.M., and Miyauchi, M.Y.H. 2011. PGPR in “coniferous trees.” In Bacterial inn Agrobiology Crop Ecosystems. ed.  D.K. Maheshwari Berlin Springer, pp. 345-359.
Che Y, Rekha PD, Arunshen AB, lai WA and Young CC, 2006. Phosphate solubling bacteria from subtropical soil and their tricalcium phosphate solubilizing abilituies. Appl. Soil Ecol. 34:33-41.
Doss Dayal, D Archana GM and Mahadevaswamy, 2008. Effect of PGPR on growth parameters in exotic Medicinal plants Pulicaria species. J. Soil Biol.Ecol. 28 (1&2) : 116-121.
Fellbaum CR, Mensah JA, Cloos AJ, Strahan ge, Pfeffer PE, Kiers ET Bucking H, 2014. Fungal nutrient allocation in common mycorrhizal networks is regulated by the carbon source strength of in dividual host plants, New Phytol. 203; 646-656:
Gaur A and Adholea A, 1994. . Estimation of VAM Spores  in the soil –A modified method. Mycorrhiza News, 6:110-11.
Genderman JW and Nicholoson  H, 1963. Spores of mycorrhizal endogone species extracted  from soil by wetsieving and  decanting.  Trans. British Mycol. Soc. 46: 235-244.
Glick BR, 1995. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology, 41:109-117.
Gryndler M, 2000 . Interactions of AM fungi  with other soil organisms. In: Kaoulink Y. and Douds D.D.  Jr. (eds.) AM Physiology  and function. pp.239-262.
Hee ZL, Bian W and Zhu J, 2002. Screening and Identification of micro-organisms capable of utilizing  phosphate absorbed  by goethite  community.  Soil Sci. Plant anal. 33:647-663.
Herter S, Schmidt M, Thompson MKL, Mikollasch A and Schauer F, 2011. A New phenol oxidase produced during melonogenesis  and encystment  stage in the nitrogen –fixing soil  bacterium  Azotobacter chroococum . Applied  Microb.  Cell. Physiol. 90.1037.- 1049.
Hosmani PA, Lakshman HC, Kolkar KP and Godi SB, 2013. Synergistic Effect between AM Fungus and Rhizobium on Cajanus cajana. In proceedings of National Conf. on  Biotechnology. pp.96-99.
Jalaluddin Hamid M, 2011. Effect of adding Inorganic organic and microbial fertilizers  on seed germination  and seedling growth of sunflower. Pakistan Journal of Botany, 40:391-396.
Johnnson Paul JF, Finlay LRRD, 2004. Microbial interactions in the mycorhizzospare  and their significance for sustainable agriculture. FEMS Microbiol. Ecol. 48:1-13. 
Jyothi P and Lakshman HC, 2017. Effect of AM fungi, Azospirillum brasiloise and pseudomonas fluorescence  on growth, bio mass, nutrient uptake in cultivar Byadagikaddi of Capsicum annum L. In J. Pharma. Bio Sci. 8(1): 704-712.
Kalavathi BP, Santhanakrishna P and Divya MP, 2000 Effect of AM fungus and phosphorus solubiling bacteria in neem. Indian Forester. 126(1): 67-70.
Kucey RMN, 1983. Phosphate Solubilizing bacteria and fungi in various cultivated in virgin Alberta soils. Canadian Journal of Soil Science, 63:671-678.
Lakshman HC and Romana RM, 2014. Beneficial effect of PSB and AM Fungus on  growth and P uptake in  Soyabean Var LSbl. In: Proceeding of Nat. Con. Held at Gadag K.L.E. College I  September 5 to 6. pp.67-70.
Lakshman HC, Ratageri RH, Rolli NM and Nadagouda MG, 2006. Interaction between Azospirillum and Phosphate solubiling bacteria (Bacillus polymyxa) on the yield of  Capsiumnnum L. (Chilli). J. Theo. Expt. Bio.  2  3 and 4: 133-135.
Lakshman, H.C. VA-mycorrhizal Studies on some important timber tree Species. Ph.D. Thesis Karnatak University, Dharwad – 580003, India.249 pp.
Linderman RG, 1988. Mycorrhizal interaction with rhizosphere  microflora. The mycorhizosphere  effect. Phytopathology, 78:366-371).
Liu RJ, Dai M, Wu X. Li. M. Liu XZ. 2012a. Suppression of the root-knot nematode (Meloidogyne incognita (Kofoid & White, Chitwood) on Tomato by dual inoculation with arbuscular mycorhizal fungi andn plant growth promoting Rhizobacteria. Mycorrhiza. 22:289-296.
Madgaonkar SC and Lakshman HC, 2013.  Efficacy of AM fungi and Azotobacter and Phosophate Solubilizing bacteria in improving of Amaranthus Paniculatus L. A leaf vegetable. Res. J. of Biotech. 8(3):36-39.
Martin BN, Joan L and Damase PK, 2018. Mycorrhiza and Rhizobacteria on  Precambrian rocky GoldmineTailings:1.Mine-adapted symbionts promote white spruce health and Grwoth. Plant Science. 9:1-11.
Maya C and Lakshman HC, 2009. Interaction between Arbuscular  mycorrhizal fungi and Rhizobiusm and their effects on Cassia accidentali-. National Journal of  Plant Science, 6(1): 25-30.
Meng L, Zhang A, Wang Han X. Wang D US, 2015. Arbuscular Mycorrhizal Fungi and Rhizobium Facilitate Nitrogen Uptake And Transfer In  Soyabean and Maize Intercropping System. Front Plant Sci. 6:339.
Muthukumar T, Udaiyan K and Rajeshkarnan V, 2001. Response of neem to Indigenous  AMfungi, phosphate solubiliziong and asymbiotic  nitrogen-fixing bacteria  undertropical  nursery conditions. Biology and Fertility of Soils. 34(6):pp 417-426.
Pikovskaya RI, 1948. Mobilization of phosphorus in soil in the connection  with vital activity of some microbial species, Mikrobiologia. 17: 362-370.
Philips JM and jayman DS, 1970. Improved procedure for cleaning roots and staining parasitic and vescular mycoorizal fungi for rapid assessment of infection. Trans. Brit. Myccl. Scc.53: 158-161.
Ramakrishnaiah G and Vijaya T, 2013. Influenmce of AM fungi Azotobacter spp.  And PSB on soil phosphatase activity and nutrients (N,P,K, Cu, Zn, Fe and Mn) status in therhizosphere of Stevia Rebaudianam (Bert.) plants. American Journal of Plant Sciences.pp. 1443-1447.
Shweta J Sabbannavar  and Lakshman HC, 2008. Interaction between Azotobacter  Pseudomonas and Fungi on two varieties of Sesamum indica L.J. Agronomy and Crop Science. 194 (6): 472-478.
Smith SE and Read DJ, 1997. Mycorhizal symbiosis SanDiego CA, USA, Academic Press, 408 pp..
Sokorich, D. 1992. Achievements and future directions of Sunflower breeding. Field crops Res. 30:231-270.
Vaddar Umeshm, Patil AB and Goudar Geeta, 2009. Isolasation and screening of  nitrogen fixers and phosphorus solubilizers from grape rhzosphere.  J. Soil. Biol .Ecol. 29 (1&2):1-12.

How to cite this article

Kamble SS and HC Lakshman, 2019. Positive effect of Am fungi and pgpr on growth Biomass yield of (sunflower) Helianthus annus Lsh1 var. Bioscience Discovery, 10(4):156-164.

Note: The editor, owner, printer and publisher does not accept any responsibility regarding unsolicited publication material, authenticity of data, statement, inaccurate data, opinions, results and view expressed by the authors in articles are of their own and not of Bioscience Discovery Journal.

Online available on


http://www.jbsd.in
http://www.biosciencediscovery.com

Publisher: RUT Printer and Publisher, Jalna (MS) India