Spectrum and frequency of Chlorophyll mutation in grass pea (Lathyrus sativus L)
Kulkarni Ganesh. B.
Associate professor in Botany, J.E.S. R.G. Bagdia Arts, S.B. Lakhotia Commerce and
R. Bezonji Science College, Jalna 431203 (M.S.), India
The seeds of grass pea (Lathyrus sativus L.) various chemical mutagen concentrations (EMS 0.05%, 0.10%, 0.15%; SA 0.005%, 0.010%, 0.015% and NMU 0.15%, 0.30%, 0.45%) and physical mutagen doses (Gamma rays- 15KR, 30KR, 45KR) were treated in laboratory. The Treated and untreated seeds were sown in the field by CRBD method. The growth of M1 generation observed the characters and number of mutants in each population thoroughly. The chlorophyll mutation frequency was calculated on the basis of plant population with explicit chlorophyll mutation and leaf phenotypic diversity. A noticeable varied range of chlorophyll and morphological mutants were observed in the M1 generations. Distinct kinds of spectrum of chlorophyll mutants’ viz. xantha, viridis, albino and chlorina were identified in the treated population. Prominent phenotypic aberrations were also noted viz. reduction in size of leaves, obtuse and retuse apex, notch at margin, bilobed leaf, lanceolate leaf, wrinkled leaflet and triforked leaf, etc. The frequency of leaf morphological changes observed in almost all chemical and physical mutagen in grass pea.
Bara BM Chaurasia AK and Verma P, 2017. Gamma rays effect on frequency and spectrum of chlorophyll mutation in chickpea (Cicer arietinum L.). J of Pharmaco and Phytochem, 6 (3): 590-591
Bhat TA, Khan AH and Parveen S, 2007. Spectrum and frequency of chlorophyll mutation induced by MMS, Gama rays and their combination in two varieties of Vicia faba L. Assian J Plant Sciences 6 (3):558-561.
Bradley MS, 1999. Legume genetic resources with novel value added industrial and pharmaceutical use. In: perspective on new crops and new uses (ed. Janik J.) Aslts press. Alexandria pp. 196-201.
Das TR and Baisakh B, 2010. Effects of mutagenic treatment on chlorophyll mutation in green gram (Vignaradiata L. Wilczek). e-planet 9 (2) : 17 – 20.
Deka RK, and Sarkar CR, 1990. Nutrient composition and antinutritional factors of Dolichos lablab L seeds. Food chem, 38: 239-246.
FAO 2002 (Ochatt S J, Muneaux E, Machado C J, L, Pontécaille C) The hyperhydricity of in vitro regenerants of grass pea (Lathyrus sativus L.) is linked with an abnormal DNA content J plant physio 159 (9), 1021-1028.
Gelin OEA, 1954. X-ray mutants in peas and vetches. Act Agr. Scand. 4: 558-568.
Girija M and Dhanavel D, 2009. Mutagenic Effectiveness and Efficiency of Gamma Rays, Ethyl Methane Sulphonate and their Combined Treatments in Cowpea (VignaunguiculataL. Walp) Global J Mol Sci, 4 (2): 68-75.
Goyal S and Khan S, 2010. Induced muatagenesis in Urdbean (Vigna mungo L. Hepper): a Review, Int. J. Bot.6(3):194-206.
Gustafsson A, 1940. The mutation system of the chlorophyll apparatus. Lund Univ. Arsskriftm N.F.Avd2 Bd 36(11) Kungliga Fysiografiska Sallskapets Handi N.F.Bd 51(11):3-40.
Khan S Wani M R Bhat M and Parveen K, 2005. Induced Chlorophyll Mutations in Chickpea (Cicer arietinumL.) Int J Agric and Bio l7 (5): 764–767.
Kharkwal MC, 1998. Induced mutations in chickpea (Cicer arietinum L.) Frequency and spectrum of chlorophyll mutations. Int J Genet, 58: 465-474.
Konzak CF Nilan R A Wagner J and Foster RJ, 1965. Efficient chemical mutagenesis, the use of induced mutations in plant breeding. Rep. FAO/IAEA Tech. Meeting, Rome, Pergamon press, pp. 49-70.
Kulkarni GB and Mogle UP, 2013. Effects of mutagen on chlorophyll mutation in horse gram [macrotyloma uniflorum (Lam) Verdcourt] Bioscience Discovery, 4 (2):214-219.
Kumar A Parmhansh P and Prasad R, 2009. Induced chlorophyll and morphological mutations in Mungbean (Vigna radiata L. Wilczek). Legume Res. 32 (1): 41- 45.
kumar AV Ushakumari R Vairam N and Amutha R, 2010. Effect of physical mutagen on expression of characters in arid legume pulse cowpea (Vigna unguiculata (L.)Walp.) J of Plant Breeding, 1(4): 908-914.
Lal GM Toms B and Smith S, 2009. Induced Chlorophyll Mutations in Black Gram, Asian J Agri. Sci. 1(1): 1-3.
Mahadevu P and Byregowda M, 2005. Genetic improvement of Dolichos bean (Lablab purpureus L.) through the use of exotic and indigenous germplasm. Indian J. Plant Genet. Resour. 18: 1-5.
Mahamune SE And Kothekar VS, 2012. Induced chemical and physical mutagenic studies in M1 generation of French bean(Phaseolus vulgaris L.) Current Botany, 3 (3): 17-21.
Monika S and Seethraman N, 2017. Induced chlorophyll and viable mutation in Lablab purpureus (L.) sweet Var. typicus through gamma rays and Ethyl Methane Sulphonate. Int J Pharm Bio Sci 8 (2): (B) 240 -249.
More SB Kulthe M Kulthe M Kashid NG and Kothekar VS, 2011. Effect of EMS and SA in Chickpea (Cicer arietinum L.) Bioscience discovery 2(1): 82-84.
Prasad AB and Das AK, 1980. Studies on induced chlorophyll mutations in Lathyrus sativus L. Cytologia, 45: 335-341.
Sangsiri C, Sorajjapinun W and Srinives P 2005 Gamma radiation induced mutations in Mungbean. Science Asia 31: 251-255.
Satpute RA, 1994. Mutation studies in Safflower (Carthamus tictorious L.) Ph.D Thesis, Dr. B.A.M.U., Aurangabad.
Sharma SK and Sharma B, 1981. Induced chlorophyll mutation in lentil. Indian J. Genet., 41: 328-333.
Singh GR, Sareen PK And Saharan RP, 2000. Induced chlorophyll and morphological mutations in Mungbean. Indian J Genet, 60(3): 391-393.
Smartt J, 1984. Evolution of grain legumes.I. Mediterranean Pulses. Exp. Agric. 20: 275-296.
Talukdar D Biswas SC and Biswas AK, 2001. Induced mutation in grass pea (Lathyrus sativus L). Prespespective in Cytology and Genetics, 10:481-484
Talukdar D, Biswas AK, 2005a. Multiple alleles controlling stipule characters in grass pea (Lathyrus sativus L.). In: Kharkwal MC (eds) Proc. 4th Intl. Legume Conference held at New Delhi, India, ISGPB publication, pp. 200.
Talukdar D Biswas AK, 2005b. Induced seed coat colour mutations and their inheritance in grass pea (Lathyrus sativus L.). Indian J. Genet. 65: 135-136.
Thilagavathi C and Mullainathan L, 2009. Isolation of Macro Mutants and Mutagenic Effectiveness, Efficiency in Black Gram (Vignamungo(L.) Hepper) Global J of Mol Sci, 4 (2): 76-79.
Undirwade GP and Kulkarni GB, 2018. Effectiveness and efficiency of mutagen on leaf chlorophyll in Hyacinth bean Lablab purpureus (L.) Sweet Int. J. of Life Sciences, 6 (1):111-116
Vikram N Ibrahim S M and Vanniarajan C, 2014. Frequency and spectrum of chlorophyll mutations in green gram [Vigna radiata (L.) Wilczek] Asian J of Bio Science, 9 (2): 204-207.
Waghmare VN and Mehra RB, 2001. Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrus sativus L. Ind J Genet 58: 465-474.
Zacharias M, 1956. Mutations verscheankultir flanzen VI. Rentgen bestranlungender Sosabohne (Glycinesoja L.) Zilcchter 26: 321-328.
How to cite this article
Kulkarni Ganesh. B., 2019. Spectrum and frequency of Chlorophyll mutation in grass pea (Lathyrus sativus L).. Bioscience Discovery, 10(4):214-221.
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